Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells.
Identifieur interne : 002685 ( Main/Exploration ); précédent : 002684; suivant : 002686Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells.
Auteurs : Ho Jin Heo [États-Unis] ; Chang Yong LeeSource :
- Journal of agricultural and food chemistry [ 0021-8561 ] ; 2005.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Anthocyanins, Phenols.
- chemical , pharmacology : Anthocyanins, Hydrogen Peroxide.
- chemistry : Citrus sinensis, Fragaria, Fruit, Musa.
- drug effects : Apoptosis.
- physiology : Oxidative Stress.
- Animals, PC12 Cells, Rats.
Abstract
Because strawberries are known to contain higher concentrations of phytochemicals and have higher antioxidant capacity among common fruits, their neuroprotective activity was tested in vitro on PC12 cells treated with H2O2. Their protective effect and antioxidant capacity were also compared with those of banana and orange, which are the fresh fruits consumed at highest levels in the United States. The cell viability test using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed that strawberry phenolics significantly reduced oxidative stress-induced neurotoxicity. Because oxidative stress is also known to increase neuronal cell membrane breakdown, lactate dehydrogenase (LDH) and trypan blue exclusion assays were also performed. Strawberry showed the highest cell protective effects among the samples. The overall relative neuronal cell protective activity of three fruits by three tests followed the decreasing order strawberry > banana > orange. The protective effects appeared to be due to the higher phenolic contents including anthocyanins, and anthocyanins in strawberries seemed to be the major contributors.
DOI: 10.1021/jf048616l
PubMed: 15769124
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000D22
- to stream PubMed, to step Curation: 000D22
- to stream PubMed, to step Checkpoint: 000D22
- to stream Ncbi, to step Merge: 000364
- to stream Ncbi, to step Curation: 000364
- to stream Ncbi, to step Checkpoint: 000364
- to stream Main, to step Merge: 002798
- to stream Main, to step Curation: 002685
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells.</title><author><name sortKey="Heo, Ho Jin" sort="Heo, Ho Jin" uniqKey="Heo H" first="Ho Jin" last="Heo">Ho Jin Heo</name><affiliation wicri:level="4"><nlm:affiliation>Department of Food Science and Technology, Cornell University, Geneva, New York 14456, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science and Technology, Cornell University, Geneva, New York 14456</wicri:regionArea><orgName type="university">Université Cornell</orgName><placeName><settlement type="city">Ithaca (New York)</settlement><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Lee, Chang Yong" sort="Lee, Chang Yong" uniqKey="Lee C" first="Chang Yong" last="Lee">Chang Yong Lee</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15769124</idno><idno type="pmid">15769124</idno><idno type="doi">10.1021/jf048616l</idno><idno type="wicri:Area/PubMed/Corpus">000D22</idno><idno type="wicri:Area/PubMed/Curation">000D22</idno><idno type="wicri:Area/PubMed/Checkpoint">000D22</idno><idno type="wicri:Area/Ncbi/Merge">000364</idno><idno type="wicri:Area/Ncbi/Curation">000364</idno><idno type="wicri:Area/Ncbi/Checkpoint">000364</idno><idno type="wicri:doubleKey">0021-8561:2005:Heo H:strawberry:and:its</idno><idno type="wicri:Area/Main/Merge">002798</idno><idno type="wicri:Area/Main/Curation">002685</idno><idno type="wicri:Area/Main/Exploration">002685</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells.</title><author><name sortKey="Heo, Ho Jin" sort="Heo, Ho Jin" uniqKey="Heo H" first="Ho Jin" last="Heo">Ho Jin Heo</name><affiliation wicri:level="4"><nlm:affiliation>Department of Food Science and Technology, Cornell University, Geneva, New York 14456, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Food Science and Technology, Cornell University, Geneva, New York 14456</wicri:regionArea><orgName type="university">Université Cornell</orgName><placeName><settlement type="city">Ithaca (New York)</settlement><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Lee, Chang Yong" sort="Lee, Chang Yong" uniqKey="Lee C" first="Chang Yong" last="Lee">Chang Yong Lee</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="ISSN">0021-8561</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Anthocyanins (analysis)</term><term>Anthocyanins (pharmacology)</term><term>Apoptosis (drug effects)</term><term>Citrus sinensis (chemistry)</term><term>Fragaria (chemistry)</term><term>Fruit (chemistry)</term><term>Hydrogen Peroxide (pharmacology)</term><term>Musa (chemistry)</term><term>Oxidative Stress (physiology)</term><term>PC12 Cells</term><term>Phenols (analysis)</term><term>Rats</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Anthocyanins</term><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anthocyanins</term><term>Hydrogen Peroxide</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Fragaria</term><term>Fruit</term><term>Musa</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>PC12 Cells</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Because strawberries are known to contain higher concentrations of phytochemicals and have higher antioxidant capacity among common fruits, their neuroprotective activity was tested in vitro on PC12 cells treated with H2O2. Their protective effect and antioxidant capacity were also compared with those of banana and orange, which are the fresh fruits consumed at highest levels in the United States. The cell viability test using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed that strawberry phenolics significantly reduced oxidative stress-induced neurotoxicity. Because oxidative stress is also known to increase neuronal cell membrane breakdown, lactate dehydrogenase (LDH) and trypan blue exclusion assays were also performed. Strawberry showed the highest cell protective effects among the samples. The overall relative neuronal cell protective activity of three fruits by three tests followed the decreasing order strawberry > banana > orange. The protective effects appeared to be due to the higher phenolic contents including anthocyanins, and anthocyanins in strawberries seemed to be the major contributors.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><noCountry><name sortKey="Lee, Chang Yong" sort="Lee, Chang Yong" uniqKey="Lee C" first="Chang Yong" last="Lee">Chang Yong Lee</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Heo, Ho Jin" sort="Heo, Ho Jin" uniqKey="Heo H" first="Ho Jin" last="Heo">Ho Jin Heo</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002685 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002685 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15769124
|texte= Strawberry and its anthocyanins reduce oxidative stress-induced apoptosis in PC12 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15769124" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |